The Selecao go into their final group-stage match with at least three absentees.
Both Neymar and Alex Sandro will miss Brazil’s upcoming match against Cameroon, according to Selecao’s team doctor.
The pair join Danilo, who will stay on Brazil’s injury list with an ankle injury.
Neymar suffered a similar injury in the 80th minute of Brazil’s win over Serbia last week. The Paris Saint-Germain star won’t be able to play for the Selecao before the last-16 round.
Alex Sandro will also miss the Cameroon game after suffering a hip injury in his side’s narrow win over Switzerland on Monday.
“After yesterday’s game, Alex Sandro felt pain in his left hip and was unable to continue in the game,” team doctor Rodrigo Lasmar said on Tuesday.
“This morning he was reassessed. We took him for an MRI test, which showed a muscle injury in the left hip muscle. The player will not be able to participate in the next match.
“But he is still undergoing treatment so that we can recover him as soon as possible.
“Danilo and Neymar are still recovering from ankle injuries. Each with a different treatment because they are different injuries.”
In case they qualify for the next round as the group leaders, Brazil will face the Group H runner-ups.
Ghana is currently staying behind Portugal as the group leaders. But South Korea and Uruguay still have a chance to leapfrog them on the final day.