The Liverpool right-back was forced off the pitch after picking up a knock while on international duty on Wednesday night.
Trent Alexander-Arnold will not be representing England in this summer’s European tournament.
Alexander-Arnold limped off late in the second half in England’s 1-0 friendly win over Austria after suffering a thigh injury.
A medical assessment has now revealed a grade two quadricep tear for the defender, meaning he will remain sidelined for a period of between four to six weeks.
The 22-year-old has been, as a result, withdrawn from the Three Lions squad, with manager Gareth Southgate expected to confirm a replacement for the injured player within the next three days.
Southgate could name one of Aaron Ramsdale, James Ward-Prowse, Jesse Lingard, Mason Greenwood, Ben White, Ben Godfrey, and Ollie Watkins in the Euro 2020 squad as the inclusion of Keiran Trippier, Reece James, and Kyle Walker means he already has enough options for the right-back spot.
An official statement released by the England camp read: “Trent Alexander-Arnold has been ruled out of UEFA EURO 2020.
“The Liverpool player sustained a thigh injury in the closing stages of Wednesday’s 1-0 win against Austria in Middlesbrough.
“A scan on Thursday confirmed the 22-year-old will be unable to play in the forthcoming tournament so he’s withdrawn from the England squad.
“Gareth Southgate will not confirm a replacement until after Sunday’s return to the Riverside Stadium for the warm-up fixture against Romania.”